The Paris 2024-bound athlete proved that he's on top form just days out from this summer's Olympic Games with a blistering time of 43.74 seconds in front of a home crowd at the London Stadium, as fellow Team GB athlete Charlie Dobson finished fourth in a time of 44.23.
Hudson-Smith smashed his previous European record and personal best of 44.07 that he set back in May at Bislett Stadium in Oslo and will now head to Paris as the fastest man in the world over 400m this year.
It was a meeting of falling records and fast times as a stunning five world leads and seven meeting records lit up the London Stadium.
Keely Hodgkinson elevated her previous women's 800m world lead to a time of 1:54.61.
Hodgkinson led home a British top three as fellow Paris 2024-bound athletes Jemma Reekie and Georgia Bell finished in second and third respectively.
Building on a calm and composed opening lap, Hodgkinson and Reekie kicked on before the Tokyo 2020 Olympic silver medallist stormed ahead for the win in a new British record and personal best time with Reekie and Bell also recording personal bests.
In the women's 4x100m, Dina Asher-Smith, Daryll Neita, Amy Hunt and Imani-Lara Lansiquot also set a new British record of 41.55 seconds.
Asher-Smith led out the quartet, with Lansiquot and Hunt extending the lead before Neita brought it home for the victory.
It was a new world lead for the quartet who smashed the previous time of 41.85 seconds from the USA, before Asher-Smith and Neita were back on the track to record season's best times in the 200m.
Away from the British action, the Netherlands' Femke Bol clinched a new 400m hurdles meeting record in a time of 51.30.
Bol had previously revealed that she was feeling the strongest she ever had in the lead-up to Paris 2024 and feeling turned into fact as she now heads into her second Olympics as one to watch.
Jamaica's Nickisha Pryce set a new world lead in the women's 400m in a roaring time of 48.57 and Switzerland's Dominic Lobalu also set a new national record in the men's 3000m.
With less than one week to go until Paris 2024, the man everybody knows, Noah Lyles rocketed to a personal best of 9.81 (-0.3) seconds to successfully defend his men's 100m London Diamond League title.
Elsewhere, the USA's Gabby Thomas took women's 200m victory with a well-timed dip on the line to deny Saint Lucia's Julien Alfred.
Earlier in the session, Brits lit up the track in the para events as Mel Woods finished third in the women's 800m wheelchair race for a new T54 British record of 1:46.85.
Sammi Kinghorn was first to the line for victory whilst Nathan Maguire stormed the men's 1500m wheelchair field.
The men's ambulant 100m was a thriller with Zak Skinner pipping fellow Brit Zac Shaw to the line in a new British T13 record and personal best of 10.84.
The London Athletics Meet will return to the London Stadium next year on Saturday 19th July. Be first in line for tickets when they go on sale. Register your details now at britishathletics.org.uk/london-athletics-meet-2025 and get priority access to secure the best seats before anyone else.
